Technical Seminar Sponsored by Intabio

Imaged capillary isoelectric focusing (iCIEF) is widely used for protein charge heterogeneity analysis during development and manufacturing of biotherapeutic drugs. Although iCIEF can indicate the presence of a charge isoform at a specific isoelectric point, it generally cannot provide molecular structure information. To address this analytical need, our group at Pfizer initiated a multi-month project to evaluate a novel iCIEF-MS platform recently described by Mack and coworkers at IntabioRef1. By using microfluidic chip-based technology, the Blaze system directly couples iCIEF with MS analysis via an on-chip ESI tip, enabling MS spectra to be collected for iCIEF peaks. We will report results from our evaluation of this iCIEF-MS system using a variety of mAb test samples.