The Blaze system directly couples high-resolution MS analysis with imaged capillary isoelectric focusing
(iCIEF) to enable monitoring of multiple critical quality attributes in a single 15-minute iCIEF-MS assay. In
this Technical Seminar, we will present details of the Blaze system’s design, including a novel nebulizer
chip design. Detailed characterization of charge variants by chip-based electrospray ionization and
interfacing to multiple MS systems including SCIEX, Thermo and Bruker will be presented. Comparative
data on the Blaze system compared to legacy cIEF will also be shown. A new, streamlined software
workflow from Protein Metrics for Blaze will demonstrate the combined analysis of iCIEF and MS data.
The latest results from our biopharmaceutical collaborators demonstrate use of Blaze to monitor a wide
variety of mAb structural variants.