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Development and Assessment Of Empty and Full Adeno-associated Virus by Imaged Capillary Isoelectric Focusing - Technical Seminar Sponsored by ProteinSimple, A Bio-Techne Company

‐ Jun 7, 2021 11:35am

The separation of Adeno-associated virus (AAV) Empty and Full by charge-based techniques have been development on several serotypes using ion-exchange chromatography. However, the separation is quite serotype dependent and sometimes process dependent on other factors. Another technique is needed to separate hard-to-separate AAV in order to characterize the empty species. Here we will explore the use of ICIEF to separate a previously failed-to-separated AAV drug substance, and present the method development and assessment of empty species. Talk will cover:
-Introduction of iCIEF and empty/full separation.
-Development of empty and full characterization by iCEIF.
-What has the empty/full characterization by iCIEF tell us.