Both the EMA and FDA recognize the vital importance of the Higher Order Structure (HOS) of a protein. It is specifically called out in their guidances and it is their expectation that the latest technology be applied to understand it. Nuclear Magnetic Resonance Spectroscopy (NMR) is often overlooked, as too complex or too costly, when people plan their HOS analyses. However, NMR is an extraordinarily well understood technique that has been shown to be highly precise, robust, and reproducible. In fact, 1-dimensional and 2-dimensional NMR data are so information rich that a bold applicant could use it as their sole HOS technique. This talk will discuss NMR (and other HOS techniques) and how to put together a rock solid characterization package.