Technical Seminar - Innovations in Capillary Electrophoresis – Maurice Platform Based In-depth Characterization of Protein Charge Variants and Fast and High Throughput High Resolution Protein Size Analyses

Sponsored by ProteinSimple, a Bio-Techne Brand

The first segment of the technical seminar will focus on an innovative fractionation workflow. A thorough characterization of a biopharmaceutical drug candidate is critical during development to attain the ultimate goals of commercializing a safe and efficacious therapeutic for patients. An important regulatory requirement is to characterize charge variants. The traditional technique used by Maurice icIEF users is to develop an ion exchange chromatography assay, collect fractions and analyze them using a mass spectrometer. ProteinSimple has developed a fractionation capable icIEF solution and is collaborating with 908 Devices in charge variant characterization using their ZipChip device connected to a mass spectrometer, providing a complete workflow for charge variant analysis. This solution offers a powerful charge variant characterization tool for biotherapeutic characterization. The presentation will include the analysis of the NIST mAb along with a second monoclonal mAb and will present icIEF fractionation and characterization data.
The second segment will discuss a unique fast and high throughput CE-SDS Maurice capability. Scientists currently use techniques that can require up to 45 minutes per injection to be able to analyze a sample and achieve excellent resolution. The innovative Turbo CE-SDS™ Cartridge for Maurice provides results in less than 6 minutes (up to a 75% time reduction from some traditional assays) and provides similar profiles and % area values compared to traditional methods. Reproducibility is excellent with CVs as low as 0.05%. This new cartridge, which runs on Maurice after a simple software upgrade, enables analysis of up to 144 samples in 8 hours across 2 batches, with up to 96 injections per batch. This high throughput CE-SDS cartridge will enable the screening of a large number of samples on CE-SDS methods addressing the sample bottle-neck challenge that customers are currently facing. The speed and data quality also make it ideal for running a much smaller number of samples.
Attendees will learn about:

o The new charge variant characterization solution, which offers workflow enhancements
o How the new Turbo CE-SDS™ cartridge for Maurice provides a fast and high throughput capability