With the growing demand for biotherapeutics, particularly those based on in vitro transcribed (IVT) mRNA and adeno-associated virus (AAV) vectors, global plasmid DNA production has surged over the past five years. This increase highlights the need for high-throughput, reliable analytical tools to support scalable biomanufacturing.

Built on the robust capabilities of Agilent’s Fragment Analyzer systems, the new Plasmid Topology Kit offers a fast, accurate, and reproducible method for analyzing plasmid DNA. It is optimized for purity assessment and sizing of supercoiled plasmids between 2,000 and 10,000 base pairs. The assay delivers results in 45 minutes for up to 48 samples in parallel, using automated parallel capillary electrophoresis, making it the ideal choice for higher throughput labs.

With minimal sample input between 0.1 and 1 ng/µL, this assay is highly sensitive and capable of determining the purity percentage of supercoiled plasmids, a critical quality attribute in plasmid production. This enables researchers to gain deeper insights into sample integrity and streamline quality control processes. Detailed data on plasmid sizing, purity analysis, and detection sensitivity will be presented.

Attendees will gain a comprehensive understanding of the assay’s performance and its potential to enhance plasmid DNA workflows. With this launch, Agilent reinforces its commitment to innovation and scientific excellence. Join us at the seminar to discover how this powerful new assay can elevate your lab’s capabilities and support the evolving needs of modern biotherapeutic development.

For Research Use Only. Not for use in diagnostic procedures.

PR 7001-4728

Johannes Schlecht

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